SELECT GUT MICROBIOTA IMPEDE ROTAVIRUS VACCINE EFFICACYSUMMARY

Select Gut Microbiota Impede Rotavirus Vaccine EfficacySummary

Background & Aims: The protection provided by rotavirus (RV) vaccines is highly heterogeneous among individuals.We hypothesized that microbiota composition might influence RV vaccine efficacy.Methods: First, we examined the potential of segmented filamentous bacteria (SFB) colonization to influence RV vaccine efficacy in mice.Next, we probed th

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Inline Particle Size Analysis during Technical-Scale Processing of a Fermented Concentrated Milk Protein-Based Microgel Dispersion: Feasibility as a Process Control

Particle size is not only important for the sensory perception of fat-free fermented concentrated milk products, but also for processing operations because of the direct relationship with apparent viscosity.The aim of this study was to apply inline particle size analysis using focused beam reflectance measurement (FBRM) to obtain real-time informat

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Rapid Purification of Endotoxin-Free RTX Toxins

Cytolytic leukotoxins of the repeat in toxin (RTX) family are large proteins excreted by gram-negative bacterial pathogens through the type 1 secretion system (T1SS).Due to low yields and poor stability in cultures of the original pathogens, it is useful to purify recombinant fatty-acylated RTX cytolysins from inclusion bodies produced in E.coli.Su

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